IGVFFI5165MIAB

released
validated
File Set
IGVFDS8375WKPF(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)
Summary
reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinD-PCR4_S364
md5sum
85d9ed5908ba00ef968d6de9cefbbdbb
Content MD5sum
27f77915c539da63dc9a6dee2fafc83d
File Size
10 MB
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinD-PCR4_S364_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Index
TCGCTAGA:TGCG
Average Read Length
21
Read Count
430090

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI5165MIAB
Jesse Engreitz, Stanford
908 B
validated

Attribution