IGVFFI5071KMPM

released
validated
File Set
IGVFDS2620FWYA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)
Summary
reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR3_S196
md5sum
14c652242661db3c18200080bbf488a5
Content MD5sum
c8c7ea7bbf1f4075afa42d8048573bfd
File Size
8.3 MB
Submitted File Name
/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin6hrs-FF1-BinD-PCR3_S196_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Index
TGCTCGAC:GTAC
Average Read Length
21
Read Count
351446

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI5071KMPM
Jesse Engreitz, Stanford
909 B
validated

Attribution