IGVFFI5058NVSA

released

validated

File Set

IGVFDS2992RWQM(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin6hrs-FF4-BinE-PCR1_S89

md5sum

de29419adf1afd08be0376384a895d98

Content MD5sum

d2503f2075ba62703ca365386669826b

File Size

8.0 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin6hrs-FF4-BinE-PCR1_S89_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGGTCGCA:CTCT

Average Read Length

Read Count

329265

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM6864OXRH

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library