IGVFFI5037ZQHU

released

validated

File Set

IGVFDS0241XMRI(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF1-BinD-PCR1-Rep1_S4

md5sum

e0ae00d691c06c3ba09e16e5b25d2c89

Content MD5sum

d04383bac98b3db24fb2d989fec7e62e

File Size

6.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF1-BinD-PCR1-Rep1_S4_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TGCTCGAC:TTGA

Average Read Length

Read Count

311520

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5613UEVU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library