IGVFFI5010ENZG

released
validated
File Set
IGVFDS3760HQCL(CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-rna-seq-cas-ko-znf217-rep1-fastq-R2
md5sum
b48a7a9f22a7f0fa2e7f631aee216b48
Content MD5sum
092e5c51179fea316fc83ef2740d7ad7
File Size
2.4 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM73-ZNF217-1_R2_001.fastq.gz
Submitter Comment
Libraries prepared and sequenced by Genewiz. Unknown sequencing platform (best guess annotated)

Sequencing Details

Sequencing Platform
Sequencing Run
1
Lane
1
Illumina Read Type
R2
Average Read Length
150
Read Count
30025193

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.2 GB
validated

Attribution