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IGVFFI5007DWHI

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS2143BSOH(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW281_2_R1
md5sum
97e328ab8e1e612cf5ba5ff9c92edc04
Content MD5sum
0b637ceb761f7ddf92d52f49ed5c1a62
File Size
215 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-10-05-McDermott-NextSeq.10X/2023-10-05/outs/fastq_path/AAC7VLGHV/LW281_S19_L002_R1_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC7VLGHV
Lane
2
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
R1
Average Read Length
28
Read Count
9519629

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neural stem cell for 20 days (cellular sub pool: subpool_LW281) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
540 MB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
508 MB
validated