IGVFFI4992AQIL

released

validated

File Set

IGVFDS2620FWYA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinD-PCR1_S4

md5sum

a9874d0c581a469d8fff436669d2b573

Content MD5sum

1148a4cc709f7c3d0f5cd110f886aea5

File Size

4.8 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin6hrs-FF1-BinD-PCR1_S4_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TGCTCGAC:ACGT

Average Read Length

Read Count

203178

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7735WAOD

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library