IGVFFI4978WOQZ

released
validated
File Set
IGVFDS1333MBPK(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
R1 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep2-rep2-fastq-lane2-R1
md5sum
9c12a4cc5c2832670474070d8bef8e41
Content MD5sum
ffe2277d8e192e7d9f6d4cb6ab93683e
File Size
6.6 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/GEX_CRISPRi_D2_S2_L002_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
2
Lane
2
Sequencing Kit
NovaSeq 6000 S4 Reagent Kit v1.5
Illumina Read Type
R1
Average Read Length
26
Read Count
324888826

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tar
filtered feature barcode matrix
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Charles Gersbach, Duke
319 MB
validated

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI4978WOQZ, IGVFFI7428GSBY
Charles Gersbach, Duke
983 B
validated

Attribution