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INDEXED
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SequenceFile
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IGVFFI4902BJMO
IGVFFI4902BJMO
released
validated
Json view
File Set
IGVFDS9818UYJH
(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep3-BinB-PCRrep1_I2
md5sum
7659597e34dfe0f419a471336f34a514
Content MD5sum
99c50d61bca30d82600bbf2db401964a
File Size
14 MB
Submitted File Name
220228pPPIF-FFSample-4-D21-pPPIF-Amp-pPPIF-1-3-1-BinB_I2_001.fastq.gz
Sequencing Details
Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I2
Index
AGGATCTA:TGAC
Average Read Length
4
Read Count
1926675
Samples
1 item
Accession
Type
Sample Terms
Summary
IGVFSM7066SDHY
InVitroSystem
THP-1
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library
Attribution
Award
HG011972
Principal Investigator(s)
Anshul Kundaje
,
Jesse Engreitz
,
Marlene Rabinovitch
,
Lars Steinmetz
,
Michael Bassik
,
Thomas Quertermous
,
Will Greenleaf
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford