IGVFFI4866TRQV

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI4866TRQV/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI4866TRQV",
    "aliases": [
        "charles-gersbach:measurement-set-crispra-region3-1umET1-ipsc-cm-targeted-hicar-rep2-R2"
    ],
    "assay_titles": [
        "HiCAR"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG012053/",
        "component": "functional characterization"
    },
    "content_md5sum": "07c024d01951fb82eb48f77e7d1a8f55",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-10-23T19:29:42.613168+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/measurement-sets/IGVFDS3127UNFB/",
        "accession": "IGVFDS3127UNFB",
        "file_set_type": "experimental data",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM1839OFIJ/",
                "accession": "IGVFSM1839OFIJ",
                "classifications": [
                    "differentiated cell specimen"
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0009747/",
                        "status": "released",
                        "term_name": "GM25256 (WTC-11)"
                    }
                ],
                "status": "released",
                "summary": "GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 42 days, male, Homo sapiens treated with 1 μM Endothelin-1 for 3 days, transfected with a guide library",
                "targeted_sample_term": {
                    "@id": "/sample-terms/CL_0000746/",
                    "status": "released",
                    "term_name": "cardiac muscle cell"
                },
                "taxa": "Homo sapiens",
                "treatments": [
                    {
                        "@id": "/treatments/bbe5df8d-a9e2-4a99-8942-9b3b7d12c605/",
                        "purpose": "perturbation",
                        "status": "released",
                        "summary": "Treatment of 1 μM Endothelin-1 for 3 days",
                        "treatment_term_name": "Endothelin-1"
                    }
                ]
            }
        ],
        "status": "released",
        "summary": "CRISPR HiCAR integrating a guide (sgRNA) library targeting accessible genome regions in a genomic locus"
    },
    "file_size": 974160109,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI4866TRQV/@@download/IGVFFI4866TRQV.fastq.gz",
    "illumina_read_type": "R2",
    "input_file_for": [
        "/matrix-files/IGVFFI5241SJON/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/charles-gersbach/",
        "title": "Charles Gersbach, Duke"
    },
    "loci_list_for": [],
    "maximum_read_length": 100,
    "md5sum": "2193cf4edcaf4b36760da30bee0049c1",
    "mean_read_length": 100,
    "minimum_read_length": 100,
    "read_count": 17189975,
    "release_timestamp": "2024-12-13T19:43:52.174850+00:00",
    "s3_uri": "s3://igvf-files/2024/10/23/0c81e677-2a41-4942-9d72-603bfff54322/IGVFFI4866TRQV.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_kit": "NextSeq 1000/2000 P2 Reagent Kit",
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0010963/",
        "status": "released",
        "term_name": "Illumina NextSeq 2000"
    },
    "sequencing_run": 4,
    "status": "released",
    "submitted_by": {
        "@id": "/users/3782ce69-0e9f-4b46-91f9-4578139785c7/",
        "title": "Ruhi Rai"
    },
    "submitted_file_name": "/hpc/group/gersbachlab/tda17/IGVF_data_files/HiCAR/fastq/crispra_human/crispra_hicar_human_fastq/H_vp64_r2_TA448_2_1uMET1_ds.2cb990959235460a9894f1a50e0bbfdb/H_vp64_r2_TA448_2_1uMET1_S9_L001_R2_001.fastq.gz",
    "summary": "R2 reads from sequencing run 4",
    "upload_status": "validated",
    "uuid": "0c81e677-2a41-4942-9d72-603bfff54322"
}