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IGVFFI4862YBMJ

released
validated
File Set
IGVFDS3198NALA(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep2-FFrep2-BinE-PCRrep2_I1
md5sum
6a398ea67093fd18c0b98c7d9e3d5295
Content MD5sum
781a19556e70604b010978ee9e5fddd7
File Size
14 MB
Submitted File Name
220228pPPIF-FFSample-8-D21-pPPIF-Amp-pPPIF-2-2-2-BinE_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I1
Index
GTCCACAG:TGAC
Average Read Length
8
Read Count
1714211

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 80-90% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford