IGVFFI4858BKOD

released

validated

File Set

IGVFDS6238DNAR(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF1-BinD-PCR4_S106

md5sum

39ab32601dae4d1fb25b0fb84251ccd6

Content MD5sum

f4ba1b9fbe7a8a6cf6322e2610806a25

File Size

28 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF1-BinD-PCR4_S106_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GGTCCAGA:ATTG

Average Read Length

Read Count

1149828

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1425VIPO

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library