IGVFFI4854VPOB

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS4916ZYLQ(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_CAAC-THP1-BioRep2-FFrep1-BinD-PCRrep2_I1
md5sum
746c924180c0cbef9679a6d7ffb32012
Content MD5sum
a363302b51fa6a50c964b9a6c5f2b16b
File Size
12 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C11-Amp_PPIF_5splicesite-BioRep2-FFrep1-BinD-PCRrep2_S882_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
I1
Index
AGGATCTA:TATGAGTA
Average Read Length
8
Read Count
1740995

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution