IGVFFI4834NWWI

released

validated

File Set

IGVFDS3113JRZB(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF2-BinF-PCR4-Rep1_S282

md5sum

a0f2d23588914dfc266e3bd64d9c73fe

Content MD5sum

80b193adc47a86222c7a74ea204acc6e

File Size

39 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF2-BinF-PCR4-Rep1_S282_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TTGTCTAT:GCCA

Average Read Length

Read Count

1791329

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM6932DXSV

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library