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IGVFFI4720GEPB

released
validated
File Set
IGVFDS0416TFEL(CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in GATA3)
Summary
R1 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-rna-seq-cas-ko-gata3-rep2-fastq-R1
md5sum
f012e1e0f40c15e66f4cab4569ad4fb6
Content MD5sum
03c9d9cb0b5372e43dce69b1b235de4d
File Size
2.3 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM73-GATA3-2_R1_001.fastq.gz
Submitter Comment
Libraries prepared and sequenced by Genewiz. Unknown sequencing platform (best guess annotated)

Sequencing Details

Sequencing Platform
Illumina NovaSeq 6000
Sequencing Run
2
Lane
1
Illumina Read Type
R1
Average Read Length
150
Read Count
30588746

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.2 GB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke