IGVFFI4660XHJF

released

validated

File Set

IGVFDS3075NNHV(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin6hrs-FF2-BinC-PCR4_S165

md5sum

d72c31a642255c7582305975346e9e0c

Content MD5sum

1ee3c45b7bce89e897f9ea0a1c0d2b06

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin6hrs-FF2-BinC-PCR4_S165_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTATCAA:ATTG

Average Read Length

Read Count

923209

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7299CMAO

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library