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IGVFFI4650EKFW

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS3888EAKL(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSitePool-THP1-BioRep1-FFrep2-BinB-PCRrep4_I2
md5sum
0d1d8ebef2674eb71b11470889bc39f4
Content MD5sum
4c0c6f159635c009e38222103b17af3e
File Size
1.5 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_pool-Amp_PPIF_5splicesite-BioRep1-FFrep2-BinB-PCRrep4_S748_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Illumina Read Type
I2
Index
CCTTCGCA:GATATCGA
Average Read Length
8
Read Count
192215

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
variant effects
Jesse Engreitz, Stanford
pending

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford