IGVFFI4637QKGZ

released

validated

File Set

IGVFDS6282WCIA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF2-BinE-PCR1-Rep1_S233

md5sum

e6ddc759ec7d5876fd4a6d2d729a0198

Content MD5sum

9f32c048f16c416bac5205b2eafa50f8

File Size

29 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF2-BinE-PCR1-Rep1_S233_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGGTAAGG:TTGA

Average Read Length

Read Count

1348071

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0671URTU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library