IGVFFI4599HIHJ

released

validated

File Set

IGVFDS9902CYHU(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF2-BinD-PCR2_S22

md5sum

546cb74407b4b3fffc77f6c1ae42ef08

Content MD5sum

5c60a36ffb2f0235f3048f9cabb126d0

File Size

13 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF2-BinD-PCR2_S22_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTATGTT:CTCT

Average Read Length

Read Count

528193

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0606BETP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library