IGVFFI4526LHYV

released

validated

File Set

IGVFDS2217TMIN(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin6hrs-FF2-BinE-PCR1-Rep1_S149

md5sum

be12390b76645b45c59dc5b3f2832a00

Content MD5sum

b32f13163dc8b48e243d0ff84585c41d

File Size

23 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin6hrs-FF2-BinE-PCR1-Rep1_S149_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AATGTTCT:CCAG

Average Read Length

Read Count

1067843

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2128WJNF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of FAM3C) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library