{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI4525LELV/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI4525LELV",
"aliases": [
"jesse-engreitz:sequence_file-teloHAEC_2kG_library-NovaSeq1-scRNAseq_2kG_2PMDox2_4_S1_L001_R2_001.fastq.gz"
],
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "0e7502cca5bd485870925cb4835dcda0",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-21T00:21:37.252839+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/measurement-sets/IGVFDS7729SUIF/",
"accession": "IGVFDS7729SUIF",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM7987ARAX/",
"accession": "IGVFSM7987ARAX",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"status": "released",
"term_name": "TeloHAEC"
}
],
"status": "preview",
"summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "preview",
"summary": "CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci"
},
"file_size": 12355810339,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI4525LELV/@@download/IGVFFI4525LELV.fastq.gz",
"illumina_read_type": "R2",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 60,
"md5sum": "1d78dc8a9e98efa6262e56cf56cd36ec",
"mean_read_length": 60,
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"s3_uri": "s3://igvf-files/2023/12/21/c77bae6f-e48d-45f1-8c01-e0a7ddb556cd/IGVFFI4525LELV.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "NovaSeq 6000 S4 Reagent Kit v1.5",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008637/",
"status": "released",
"term_name": "Illumina NovaSeq 6000"
},
"sequencing_run": 5,
"status": "preview",
"submitted_by": {
"@id": "/users/05fa0dc9-0e33-427c-9d1f-82393edc4a03/",
"title": "Helen Kang"
},
"submitted_file_name": "/seq/landerlabCE/Users/Helen/TeloHAEC_Perturb-seq_2kG/240210_IGVF_recombined_fastqs/scRNAseq_2kG_2PMDox2_4_L001_R2_001.fastq.gz",
"summary": "R2 reads from sequencing run 5",
"upload_status": "validated",
"uuid": "c77bae6f-e48d-45f1-8c01-e0a7ddb556cd"
}