IGVFFI4503OALR

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS7850WHCP(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-FACS_Input-PCRrep1_I2

md5sum

6905f9ca8df8e6a086f9f92fabd7f3a0

Content MD5sum

4b235f0505045de3c2c22412d15911a9

File Size

15 MB

Submitted File Name

240908-S029-5splice-THP1-5splicesite_C12-Amp_PPIF_5splicesite-BioRep1-FFrep1-BinAll-PCRrep1_S987_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

1

Illumina Read Type

I2

Index

GCACATCT:AGGTGCGT

Average Read Length

8

Read Count

2248627

Samples

1 item

Accession

Type

Sample Terms

Summary

InVitroSystem

THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award

Principal Investigator(s)

Anshul Kundaje, Jesse Engreitz, Marlene Rabinovitch, Lars Steinmetz, Michael Bassik, Thomas Quertermous, Will Greenleaf

Contact P.I.

Lab

Jesse Engreitz, Stanford