IGVFFI4477VBFK

released

validated

File Set

IGVFDS8896GBSX(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinE-PCR4_S293

md5sum

37b39e4b62aa64de7f6f9a73f47390b5

Content MD5sum

1e2fe40075f7d0b643aeb8c90f598137

File Size

9.2 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin6hrs-FF1-BinE-PCR4_S293_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGCAATTC:TGCG

Average Read Length

Read Count

395166

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9116DXLY

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library