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IGVFFI4473XKFF

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS4629NPBV(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW428_4_R2
md5sum
a7322655aa802b9d38c9b5b2821af0ee
Content MD5sum
957230e27307df9d5e5bec587cf9b737
File Size
41 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon17_18/scRNA///LW428_S4_L004_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NovaSeq X Plus
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
4
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
R2
Average Read Length
151
Read Count
550009103

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW428) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
1.0 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
768 MB
validated