IGVFFI4464QAQU

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI4464QAQU/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI4464QAQU",
    "aliases": [
        "jesse-engreitz:sequence_files-TeloHAEC_Perturb-seq_2kG-Dialout_2kg_2PMDox2_2_AGGTAAGG_L001_R2_001.fastq.gz"
    ],
    "assay_titles": [
        "Perturb-seq"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "content_md5sum": "7006c38ed84e1a22ef8c6f0bfa32df82",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-02-06T21:24:25.241154+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS7359MSBY/",
        "accession": "IGVFDS7359MSBY",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM3104QEEP/",
                "accession": "IGVFSM3104QEEP",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
                        "modality": "interference",
                        "status": "released",
                        "summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/NTR_0000753/",
                        "status": "released",
                        "term_name": "TeloHAEC"
                    }
                ],
                "status": "preview",
                "summary": "TeloHAEC cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "preview",
        "summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting genes in many genomic loci"
    },
    "file_size": 377757897,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI4464QAQU/@@download/IGVFFI4464QAQU.fastq.gz",
    "illumina_read_type": "R2",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "loci_list_for": [],
    "maximum_read_length": 56,
    "md5sum": "34eba20f5a47df509cb89ef8d1c85f8c",
    "mean_read_length": 56,
    "minimum_read_length": 56,
    "read_count": 15442161,
    "s3_uri": "s3://igvf-files/2024/02/06/3a928d7c-e6c0-4c3f-acfa-f412974a9161/IGVFFI4464QAQU.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_kit": "NextSeq 500/550 High-Output v2.5 Kit",
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0009173/",
        "status": "released",
        "term_name": "Illumina NextSeq 500"
    },
    "sequencing_run": 1,
    "status": "preview",
    "submitted_by": {
        "@id": "/users/05fa0dc9-0e33-427c-9d1f-82393edc4a03/",
        "title": "Helen Kang"
    },
    "submitted_file_name": "/oak/stanford/groups/engreitz/Users/gschnitz/dialout_files_for_helen/Dialout_2kg_2PMDox2_2_AGGTAAGG_L001_R2_001.fastq.gz",
    "summary": "R2 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "3a928d7c-e6c0-4c3f-acfa-f412974a9161"
}