IGVFFI4398ITZJ

released

validated

File Set

IGVFDS2220HHTN(gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jay-shendure:CRISPRa_singlecell_iPSCneuron_VPH_sequence_CS1_10Xlane2_seqlane2_I2

md5sum

a7766730581dbe4648c2b278429a4ad9

Content MD5sum

47890b887424c4dac3d79d0084d405ae

File Size

326 MB

Submitted File Name

/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/neuron_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAMCTVHV/CS1_2/CS1_2_S6_L002_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NextSeq 2000

Sequencing Run

Flowcell ID

AAAMCTVHV

Lane

Sequencing Kit

NextSeq 1000/2000 P3 Reagent Kit

Illumina Read Type

Average Read Length

Read Count

50447615

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9913PXTT

InVitroSystem

GM25256 (WTC-11)

GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane2), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library