IGVFFI4369YDEU

released

validated

File Set

IGVFDS8719WWWS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinD-PCR4_S130

md5sum

b3b7704046e8de48bdf065d6108b4e0f

Content MD5sum

855fd78f1268da53c067bf0b33785e3a

File Size

21 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinD-PCR4_S130_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTCTGATG:ATTG

Average Read Length

Read Count

853662

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3068ZYXS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library