IGVFFI4302EURY

released

validated

File Set

IGVFDS7753HONE(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF2-BinE-PCR2_S23

md5sum

8062ecb44b0fefdc5d29b92617b3e138

Content MD5sum

7f7d4de9006179d1b5cde6a1d453fb42

File Size

7.4 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF2-BinE-PCR2_S23_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCTACCAT:CTCT

Average Read Length

Read Count

308299

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9074NPMT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library