{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI4295KZWO/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI4295KZWO",
"aliases": [
"jay-shendure:CRISPRa_singlecell_K562_VPR_sequence_CS1_10Xlane2_seqlane2_R2"
],
"assay_titles": [
"scCRISPR screen"
],
"audit": {},
"award": {
"@id": "/awards/HG011966/",
"component": "functional characterization"
},
"content_md5sum": "9b5d54027c1dbcc94688dd9eb3edbc29",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-09T00:50:38.577871+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS4834SKZS/",
"accession": "IGVFDS4834SKZS",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM9542NUGS/",
"accession": "IGVFSM9542NUGS",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/8b61bce4-105c-435e-9b32-1c8903566a9b/",
"modality": "activation",
"status": "released",
"summary": "CRISPRa Sp-dCas9-VP64-p65-Rta (VPR)"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002067/",
"status": "released",
"term_name": "K562"
}
],
"status": "released",
"summary": "K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci"
},
"file_size": 1521137303,
"flowcell_id": "AAAHG2HHV",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI4295KZWO/@@download/IGVFFI4295KZWO.fastq.gz",
"illumina_read_type": "R2",
"input_file_for": [
"/matrix-files/IGVFFI5731ZKRJ/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/jay-shendure/",
"title": "Jay Shendure, UW"
},
"lane": 2,
"loci_list_for": [],
"maximum_read_length": 91,
"md5sum": "f414c4bcbf6622b4870c55380547c65f",
"mean_read_length": 91,
"minimum_read_length": 91,
"read_count": 40042401,
"release_timestamp": "2024-08-13T20:47:08.155704+00:00",
"s3_uri": "s3://igvf-files/2023/12/09/234d65b2-49b6-4558-8817-021077cfa640/IGVFFI4295KZWO.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "NextSeq 1000/2000 P3 Reagent Kit",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0010963/",
"status": "released",
"term_name": "Illumina NextSeq 2000"
},
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/73f58e9f-8f5d-4fee-a7cd-dd63c0167e4b/",
"title": "Beth Martin"
},
"submitted_file_name": "/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/K562_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAHG2HHV/VPR_CS1_2/VPR_CS1_2_S8_L002_R2_001.fastq.gz",
"summary": "R2 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "234d65b2-49b6-4558-8817-021077cfa640"
}