IGVFFI4291ESKX

released

validated

File Set

IGVFDS0319JZYS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF1-BinA-PCR3-Rep1_S259

md5sum

05baecd4876ad2ab1d5dcf5ec764fd7a

Content MD5sum

0507526be60feb1626fcfbfb1580a3ed

File Size

30 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF1-BinA-PCR3-Rep1_S259_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AACGCATT:GCCA

Average Read Length

Read Count

1381042

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9315IOJD

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library