IGVFFI4236ZKZQ

released

validated

File Set

IGVFDS6296UIVP(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinF-PCR2_Rep2_S91

md5sum

be90bd339e35d3fab16e4fb2232ab18d

Content MD5sum

09dca2cdf8e5433a69e3b4b6023ecc6c

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinF-PCR2-Rep2_S91_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TTGAATAG:TCTC

Average Read Length

Read Count

1142651

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9724UBWS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library