IGVFFI4232HLWS

released

validated

File Set

IGVFDS6282WCIA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF2-BinE-PCR3-Rep1_S275

md5sum

eb0331bdb8c3da0c86c0797f6080bf1c

Content MD5sum

171b447877e0cf9e6feea95301905cdf

File Size

32 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF2-BinE-PCR3-Rep1_S275_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGGTAAGG:GCCA

Average Read Length

Read Count

1467181

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0671URTU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library