{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI4194ZWJO/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI4194ZWJO",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR2_Rep2_S200"
],
"assay_titles": [
"CRISPR FlowFISH screen"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "4eb03e944566c5e6f9ed15b374308da4",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T02:07:25.752390+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS1748WPZO/",
"accession": "IGVFDS1748WPZO",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM2548FJWP/",
"accession": "IGVFSM2548FJWP",
"classifications": [
"cell line"
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"term_name": "HCT116"
}
],
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"summary": "gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
"file_size": 16333787,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI4194ZWJO/@@download/IGVFFI4194ZWJO.fastq.gz",
"index": "TTACGCAC:GCCA",
"input_file_for": [
"/tabular-files/IGVFFI3437ZDNT/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 21,
"md5sum": "330dc852389bfe6d33b3bf1ee3a0f4a0",
"mean_read_length": 21,
"minimum_read_length": 21,
"read_count": 824419,
"release_timestamp": "2024-07-10T20:22:02.901177+00:00",
"s3_uri": "s3://igvf-files/2024/06/01/29e124fe-57d9-493a-860b-c09c11277090/IGVFFI4194ZWJO.fastq.gz",
"schema_version": "14",
"seqspecs": [
"/configuration-files/IGVFFI5688PYPY/"
],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008565/",
"term_name": "Illumina HiSeq 2500"
},
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/100f06c6-83a7-4b4d-9dfd-053a1829773b/",
"title": "Benjamin Doughty"
},
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinE-PCR2-Rep2_S200_R1_001.fastq.gz",
"summary": "reads from sequencing run 1",
"upload_status": "validated",
"uuid": "29e124fe-57d9-493a-860b-c09c11277090"
}