{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI4166BTNJ/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI4166BTNJ",
"aliases": [
"jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep3-BinA-PCRrep1_I1"
],
"assay_titles": [
"Variant-EFFECTS"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "5e25a2a0936e1305b3e4508fd489dc8a",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-12-05T18:59:33.924614+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/measurement-sets/IGVFDS5001PJTX/",
"accession": "IGVFDS5001PJTX",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM9234DKCR/",
"accession": "IGVFSM9234DKCR",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/fc6bc0b3-a064-47d0-b95d-a9af14bb1624/",
"modality": "prime editing",
"status": "released",
"summary": "CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0001253/",
"status": "released",
"term_name": "THP-1"
}
],
"status": "released",
"summary": "THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus"
},
"file_size": 2865444,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI4166BTNJ/@@download/IGVFFI4166BTNJ.fastq.gz",
"illumina_read_type": "I1",
"index": "GCAGCCGT:ACTG",
"input_file_for": [
"/tabular-files/IGVFFI2325NRPY/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 8,
"md5sum": "db221c75781afe995b71ba768fb0459b",
"mean_read_length": 8,
"minimum_read_length": 8,
"read_count": 360239,
"release_timestamp": "2023-12-20T23:25:58.239423+00:00",
"s3_uri": "s3://igvf-files/2023/12/05/eec63b10-7215-41a3-951b-0c4f1a227b34/IGVFFI4166BTNJ.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008566/",
"status": "released",
"term_name": "Illumina NextSeq 550"
},
"sequencing_run": 2,
"status": "released",
"submitted_by": {
"@id": "/users/f9e7cd82-a407-4a20-89ee-f4dc668b0cbe/",
"title": "Michael Montgomery"
},
"submitted_file_name": "220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep3-BinA-PCRrep1_S36_I1_001.fastq.gz",
"summary": "I1 reads from sequencing run 2",
"upload_status": "validated",
"uuid": "eec63b10-7215-41a3-951b-0c4f1a227b34"
}