IGVFFI4124LZQS

released

validated

File Set

IGVFDS1748WPZO(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF2-BinE-PCR4_Rep1_S157

md5sum

ea4a227cd9483ec04722fff278e0849d

Content MD5sum

c6435227d7304b4448495888b6123680

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF2-BinE-PCR4-Rep1_S157_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GAACCTAG:TTGA

Average Read Length

Read Count

1120128

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2548FJWP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library