{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI3970QIWD/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI3970QIWD",
"aliases": [
"charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep3-rep3-fastq-lane3-R2"
],
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "5173525523db30e5407d1a7f23e47ba3",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-11-09T19:49:18.548384+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS0591VNKJ/",
"accession": "IGVFDS0591VNKJ",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/primary-cells/IGVFSM5189UBOI/",
"accession": "IGVFSM5189UBOI",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes"
},
"file_size": 4010486942,
"flowcell_id": "HMCCWDSX3",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI3970QIWD/@@download/IGVFFI3970QIWD.fastq.gz",
"illumina_read_type": "R2",
"input_file_for": [
"/matrix-files/IGVFFI7101DZKK/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"lane": 3,
"loci_list_for": [],
"maximum_read_length": 90,
"md5sum": "297170af7d189c606def359f5fcf8324",
"mean_read_length": 90,
"minimum_read_length": 90,
"read_count": 113296238,
"release_timestamp": "2024-10-18T19:50:07.722559+00:00",
"s3_uri": "s3://igvf-files/2023/11/09/a56ba14b-30f4-4ece-8e77-856c9c7cfb28/IGVFFI3970QIWD.fastq.gz",
"schema_version": "14",
"seqspecs": [
"/configuration-files/IGVFFI6340QFOL/"
],
"sequencing_kit": "NovaSeq 6000 S4 Reagent Kit v1.5",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008637/",
"status": "released",
"term_name": "Illumina NovaSeq 6000"
},
"sequencing_run": 3,
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/gRNA_CRISPRi_D3_S9_L003_R2_001.fastq.gz",
"summary": "R2 reads from sequencing run 3",
"upload_status": "validated",
"uuid": "a56ba14b-30f4-4ece-8e77-856c9c7cfb28"
}