IGVFFI3941EFFB

released

validated

File Set

IGVFDS4662ZYNK(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF4-BinD-PCR4_S142

md5sum

7089feb4bcea08039dc51b8897568be3

Content MD5sum

2ab76db519235fe1d0b6326fa93ea024

File Size

11 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF4-BinD-PCR4_S142_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ATTGTCTG:ATTG

Average Read Length

Read Count

468409

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1961BHPA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library