IGVFFI3891DAKN

released

validated

File Set

IGVFDS7242NZGL(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinC-PCR2_S171

md5sum

773755b44f3222746505d326d691cbc6

Content MD5sum

9407babf95805430a6043903d0468220

File Size

6.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinC-PCR2_S171_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GAAGGAAG:CATA

Average Read Length

Read Count

295204

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5079ZTJX

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library