IGVFFI3880FPSK

released

validated

File Set

IGVFDS8772YTOP(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF1-BinF-PCR2_S12

md5sum

dc9e8a19501dfb16ddf2692627db1027

Content MD5sum

61c9c000f080e5d31ca469a7068263a1

File Size

15 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF1-BinF-PCR2_S12_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TTCGCTGA:CTCT

Average Read Length

Read Count

646721

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9117BTSA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library