IGVFFI3864GYTT

released

validated

File Set

IGVFDS6541EVXL(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinD-PCR3_Rep2_S95

md5sum

0876f6b0c61b63178dce62e20e8298aa

Content MD5sum

78aae67e3c36fceab2c2c8e59af00477

File Size

12 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinD-PCR3-Rep2_S95_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCAGAGCT:TCTC

Average Read Length

Read Count

627120

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9894YYOA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library