IGVFFI3801VJQA

released

validated

File Set

IGVFDS3029MTKI(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinF-PCR3_S270

md5sum

49f66f8290781d230de20678091bcaeb

Content MD5sum

9e7935e2de77d67fc0b97e600418e7bf

File Size

4.1 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinF-PCR3_S270_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CAATAGTC:GTAC

Average Read Length

Read Count

174147

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM4674ZTIR

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library