IGVFFI3797FUXG

released

validated

File Set

IGVFDS3144WFJD(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinB-PCR1_S2

md5sum

648ac80c995f3f837ebab37ea0099824

Content MD5sum

d9419120159bb0f6cd4c236f4d8c7a8d

File Size

3.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin6hrs-FF1-BinB-PCR1_S2_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CATGCTTA:ACGT

Average Read Length

Read Count

166208

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8104CCIE

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library