IGVFFI3781TWXY

released

validated

File Set

IGVFDS6449NYRM(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinB-PCR2-Rep1_S116

md5sum

6a9e93a45727c929bd729e3da1f7c48c

Content MD5sum

3da0e0741e55cc2fba67c7bebde6ec4f

File Size

17 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinB-PCR2-Rep1_S116_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CAGGAGCC:CCAG

Average Read Length

Read Count

784573

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0069TVXJ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library