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IGVFFI3777QEJF

released
validated
File Set
IGVFDS4834SKZS(gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jay-shendure:CRISPRa_singlecell_K562_VPR_sequence_CS1_10Xlane2_seqlane1_I1
External Resources
md5sum
7673861874d054e2fc8f174549d2f830
Content MD5sum
72965ef7d9e33223529f4c6b185d0e9a
File Size
319 MB
Submitted File Name
/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/K562_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAHG2HHV/VPR_CS1_2/VPR_CS1_2_S8_L001_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAAHG2HHV
Lane
1
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
I1
Average Read Length
8
Read Count
41798174

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

Files Derived From This File

3 items
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
3.3 KB
validated
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
6.9 KB
validated
tar
sparse gene count matrix
cell by gene in sparse gene count matrix
Jay Shendure, UW
538 MB
validated

Attribution

Award
HG011966
Principal Investigator(s)
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW