{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI3757DBEL/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI3757DBEL",
"aliases": [
"jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinB-PCR2_S170"
],
"assay_titles": [
"CRISPR FlowFISH screen"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "e8b04403b6f1c4b7bf5776e80462f54d",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-01T01:29:30.716573+00:00",
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS8375BGSZ/",
"accession": "IGVFDS8375BGSZ",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM7863HHJP/",
"accession": "IGVFSM7863HHJP",
"classifications": [
"cell line"
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0002824/",
"term_name": "HCT116"
}
],
"summary": "HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of CCND1) modified with a guide library",
"taxa": "Homo sapiens"
}
],
"summary": "gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci"
},
"file_size": 6560633,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI3757DBEL/@@download/IGVFFI3757DBEL.fastq.gz",
"index": "ATTCCTCT:CATA",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 21,
"md5sum": "e19946ff8fa63f12994746901ab4d028",
"mean_read_length": 21,
"minimum_read_length": 21,
"read_count": 280874,
"release_timestamp": "2024-07-10T20:22:25.672771+00:00",
"s3_uri": "s3://igvf-files/2024/06/01/042d017d-ab42-4d7f-897f-7a5d30a4a9ec/IGVFFI3757DBEL.fastq.gz",
"schema_version": "14",
"seqspecs": [
"/configuration-files/IGVFFI1109UFAS/"
],
"sequencing_platform": "/platform-terms/EFO_0008565/",
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/100f06c6-83a7-4b4d-9dfd-053a1829773b/",
"title": "Benjamin Doughty"
},
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinB-PCR2_S170_R1_001.fastq.gz",
"summary": "reads from sequencing run 1",
"upload_status": "validated",
"uuid": "042d017d-ab42-4d7f-897f-7a5d30a4a9ec"
}