IGVFFI3742WWYT

released

validated

File Set

IGVFDS4701YCEL(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinB-PCR3_Rep1_S14

md5sum

7b3cc0cd2643fad732a55c68a2baf10f

Content MD5sum

1fc0cdc0b0b82d73565eb734966b5076

File Size

36 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF1-BinB-PCR3-Rep1_S14_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CACATCCT:TGCG

Average Read Length

Read Count

1840969

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3954RGXF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library