IGVFFI3706DIIS

released

validated

File Set

IGVFDS2850LCWI(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinF-PCR1_S6

md5sum

dc4135ba387d54b84afc3569c8d5e6df

Content MD5sum

129c8c8d62119ebb837edb1553ada1a5

File Size

2.4 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin6hrs-FF1-BinF-PCR1_S6_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGTTGCTT:ACGT

Average Read Length

Read Count

100571

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0904FNLS

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library