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IGVFFI3564HSSU

released
validated
File Set
IGVFDS9108NLTS(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep1-rep1-fastq-lane2-R1
md5sum
30ccd73b882ffb95008eab2dbe857d1d
Content MD5sum
e046fbd6efd23eda223b0ace90dfc2e9
File Size
1.4 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/gRNA_CRISPRa_D1_S10_L002_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq 6000
Sequencing Run
1
Flowcell ID
HMCCWDSX3
Lane
2
Sequencing Kit
NovaSeq 6000 S4 Reagent Kit v1.5
Illumina Read Type
R1
Average Read Length
26
Read Count
65953338

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tar
filtered feature barcode matrix
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Charles Gersbach, Duke
310 MB
validated

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI9294QFQU, IGVFFI3564HSSU
Charles Gersbach, Duke
1.1 KB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke