IGVFFI3560ADOQ

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS3152TFYP(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinD-PCRrep2_I2
md5sum
83a99e0585fb0a3692e6c34e0411a594
Content MD5sum
0edf44812a3af298288588e27d519452
File Size
6.3 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C12-Amp_PPIF_5splicesite-BioRep1-FFrep1-BinD-PCRrep2_S890_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
I2
Index
CTGCGGAT:TATGAGTA
Average Read Length
8
Read Count
811533

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution