IGVFFI3552EGOZ

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS9863HSXN(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinA-PCRRep2_I2

md5sum

f83cd4edaa596b5e5028270542b8b7fa

Content MD5sum

15133e4477ba6d23b95101c72b0ec188

File Size

1.5 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-PCRRep2-BinA_S29_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

TTACGCAC:TTGT

Average Read Length

Read Count

190519

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM6795SCHR

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library